Composite

Part:BBa_K3001028:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-19)


snR30 + RNA Mango II + T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 621
    Illegal SpeI site found at 161
    Illegal PstI site found at 1080
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 556
    Illegal SpeI site found at 161
    Illegal PstI site found at 1080
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 573
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 621
    Illegal SpeI site found at 161
    Illegal PstI site found at 1080
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 621
    Illegal SpeI site found at 161
    Illegal PstI site found at 1080
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1051


Design Notes

Construct works with run-off transcription


Source

Gifted from the Kothe lab.

References